FIG. 4.

Western blot analysis of L. pentosus grown on various carbon sources. A Western blot of a sodium dodecyl sulfate–7.5% polyacrylamide gel is shown after incubation with polyclonal antibodies derived against CcpA of B. megaterium. Lane 1, 50 ng of purified CcpA of B. megaterium; lane 2, 0.2 OD₆₀₀ equivalents of protein extract of L. pentosus LPE4 (ccpA mutant); lanes 3 and 4, 0.2 OD₆₀₀ equivalents of protein extract of L. pentosus MD363 grown on M medium with 50 mM glucose and 50 mM xylose, respectively.