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. 2022 May 26;13:840719. doi: 10.3389/fimmu.2022.840719

Figure 4.

Figure 4

Endogenous IL-38 inhibits NLRP3 activation in colitis. WT and IL-38 KO (IL-38 deficient) mice subjected to DSS. (A) mRNA expression of Nlrp3 in colonic tissue measured by qPCR, normalized to GAPDH. (B) NLRP3, Caspase-1 (p20), Caspase-1 (p45) and β-Actin protein abundance in colonic tissues quantified by Western Blot analysis. Lanes 1-3 contain colonic tissue of WT mice treated with DSS, lanes 4-7 from IL-38 deficient mice subjected to DSS. A representative blot is shown from two experiments with 3-4 mice per group. (C) Quantifications of blots presented in (B). (D) P45/p20 ratio in WT mice treated with DSS compared to IL-38 KO mice. The data represents the mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, by two-way ANOVA with Fisher’s multiple comparison in (A), and student’s T-test in (C, D).