Enumeration of presumptive transconjugants in Madera Canyon soil microcosms. The data points and error bars show the means and standard deviations based on data from three replicate microcosms. No presumptive transconjugants were detected in control microcosms. Symbols: ⧫, soil amended with 500 μg of 2,4-D g of dry soil−1 and E. coli D11; ■, soil amended with 1,000 μg of 2,4-D g of dry soil−1 and E. coli D11.