TABLE 2.

Species-specific primers

Primer pair	Species	Primers	Target	Sequence (5′–3′)	PCR annealing temp (°C)	MgCl2 concn (mM)
1	L. acidophilus	Aci 16SI	16S gene	AGCTGAACCAACAGATTCAC	62	1.5
		16SII		ACTACCAGGGTATCTAATCC
2	L. crispatus	Cri 16SI	16S gene	GTAATGACGTTAGGAAAGCG	60	1.5
		16SII		ACTACCAGGGTATCTAATCC
3	L. gasseri	GasI	16S-23S spacer	GAGTGCGAGAGCACTAAAG	55	2.5
		GasII		CTATTTCAAGTTGAGTTTCTCT
4	L. johnsonii	Joh 16SI	16S gene	GAGCTTGCCTAGATGATTTTA	57	1.5
		16SII		ACTACCAGGGTATCTAATCC
5	L. plantarum	Lfpr	16S-23S spacer	GCCGCCTAAGGTGGGACAGAT	55	2.0
		PlanII		TTACCTAACGGTAAATGCGA
6	L. casei	PrI	16S-23S spacer	CAGACTGAAAGTCTGACGG	55	2.0
		CasII		GCGATGCGAATTTCTTTTTC
7	L. zeae	ZeaI	16S-23S spacer	TGTTTAGTTTTGAGGGGACG	58	2.0
		ZeaII		ATGCGATGCGAATTTCTAAATT
8	L. rhamnosus	PrI	16S-23S spacer	CAGACTGAAAGTCTGACGG	58	2.0
		RhaII		GCGATGCGAATTTCTATTATT
9	L. reuteri	Lfpr	16S-23S spacer	GCCGCCTAAGGTGGGACAGAT	55	2.0
		Reu		AACACTCAAGGATTGTCTGA
10	L. fermentum	Lfpr	16S-23S spacer	GCCGCCTAAGGTGGGACAGAT	55	3.0
		FermII		CTGATCGTAGATCAGTCAAG
11	L. sharpeae	ShaI	16S-23S spacer	GATAATCATGTAAGAAACCGC	58	1.5
		ShaII		ATATTGTTGGTCGCGATTCG