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. 2000 Jan;66(1):304–309. doi: 10.1128/aem.66.1.304-309.2000

TABLE 1.

The stability of sequences selected by in vivo selectiona

Insert Sequence design of insert Transformation efficiency (CFU/μg of DNA)
A In vivo selection 1.2 ± 0.5 × 105
B In vivo selection 0.9 ± 0.3 × 105
C In vivo selection 3.1 ± 0.9 × 105
D Codon usage <102
None 5.3 ± 1.3 × 106
a

Competent cells of E. coli HB101 (Nippon Gene, Toyama, Japan) have transformation ability of 108 CFU/μg of DNA (pBR322). Transformation was performed four times for each vector insert according to the protocol recommended by the manufacturer. Transformation efficiency values are the averages of the results of four transformation experiments. The DNA sequences of the inserts are as follows: A, GGAGCCCCGGGTGCTCCGGGTGCACCGGGTCCGGTTGGTCCGGCTGGTAAATCTGGTGACCGTGGTGAAACAGGTCCTGCAGGTCCGCCG; B, GGAGCCCCAGGTGCTCCAGGAGCACCGGGTCCAGTCGGGCCGGCAGGAAAATCAGGAGATCGCGGAGAAACAGGTCCTGCAGGACCACCA; C, GGAGCCCCAGGTGCTCCCGGAGCACCAGGACCAGTCGGGCCGGCAGGTAAATCTGGGGACCGCGGCGAGACGGGTCCTGCTGGACCACCA; and D, GGAGCCCCGGGCGCGCCCGGTGCGCCGGGACCAGTCGGGCCGGCAGGAAAATCAGGGGACCGTGGTGAAACGGGTCCTGCAGGACCACCA.