TABLE 2.
Primers used for PCR of various ORFs
| ORF | Primer
|
|
|---|---|---|
| 5′a | 3′b | |
| 1 | ATTACTCGAGGATATACCGGGAGGTAGACCAT | ATATCTGCAGTTAATCTGGCATTAATACATC |
| 1 plus tac31A | ATTACTCGAGGATATACCGGGAGGTAGACCAT | TAATCTGCAGTTACCAGAAGCCGCACATATC |
| 3 | ATATCTCGAGTGTGGAGAAAGTGAGGTGAC | GATCCTGCAGTTAAGATACATAAGAAGAC |
| 4H | GATCCTCGAGCTTAGGAGCTAAACTATGG | GTACCTGCAGTCTGACTGACGACACTTACCT |
| 5H | ATATCTCGAGGAGAGAAAGGAGCAAAAAATTGAC | GATCCTGCAGAGAAATGAGCTTCAAGAACAA |
| 6Hc | GATCCTCGAGCTGGAATTAAAGGAGCTGAA | ATATCTGCAGGTTACTATTGGCTCTTTCA |
| 6c | GATCCTCGAGCTGGAATTAAAGGAGCTGAA | ATTGCTGCAGTTATTTTTTACTTTTTAGATTC |
a
XhoI site is underlined.
b
PstI site is underlined.
c
Since the full ORF 6 sequence was not available in our laboratory, sequence information from phage r1t (50) was used to design the 3′ primers.