TABLE 3.
Comparison of the effects of expression of the various antisense cassettes from the high-copy-number vector with the effects of those from the explosive replicon on the EOP of phage φ31
| Antisense construct | EOP of phage φ31 (SD)a
|
|
|---|---|---|
| pTRKH2-based high-copy-number vector | pTRK360-based explosive replicon | |
| pTRKH2 (control) | 1.0 | — |
| pTRK360 (ori31 alone) | — | 0.36 (0.02) |
| P6::anti-ORF 1::TT7 | 0.89 (0.08) | N.T. |
| P6::anti-ORF 1 plus ORF 2::TT7 | 1.1 (0.09) | N.T. |
| P6::anti-ORF 3::TT7 | 1.3 (0.05) | 0.21 (0.04) |
| P6::anti-ORF 4H::TT7 | 1.1 (0.10) | 0.11 (0.03) |
| P6::anti-ORF 5H::TT7 | 1.1 (0.12) | 0.25 (0.02) |
| P6::anti-ORF 6H::TT7 | 1.2 (0.10) | N.T. |
| P6::anti-ORF 6::TT7 | 1.2 (0.11) | 0.33 (0.06) |
| anti-ORF 4H::TT7 (no P6) | N.T. | 0.29 (0.06) |
| P15A102x::anti-ORF 4H::TT7 | 1.1 (0.10) | N.T. |
| P15A102x::anti-ORF 4H::TT7 | 1.0 (0.13) | N.T. |
a
All EOP values were determined with L. lactis subsp. lactis NCK203 (pTRKH2) as the reference strain and are the averages of the results of at least three independent experiments. —, not applicable; N.T., not tested.