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. 2022 Jun 9;17(6):e0269547. doi: 10.1371/journal.pone.0269547

Fig 1. Conversion of primed hESCs to the naïve state of pluripotency.

Fig 1

(A) Colony morphology of primed H9 and CHA-hES4 cells and their naïve cells cultured in 2i/LF/A, 2i/L/X/F/P or LCDM media. The scale bar is 100 μm. (B, C) Cell surface expression of primed (SSE-3, SSEA-4, TRA-1-60, TRA-1-81, CD24, and CD90) and naïve markers (CD7, CD77, and CD130) on primed H9 (B) and 2i/L/F/A-naïve hPSCs. (C). Black lines, cell surface marker with the indicated primary antibodies; red area, no primary antibody control.