Figure 5.

Immunohistochemistry comparing CRVO + aflibercept vs. CRVO + NaCl. Proteome analysis showed a small downregulation of endoplasmin and PEDF, which was further explored with immunohistochemistry. Immunohistochemistry showed similar staining patterns of (A–D) endoplasmin and (E–H) PEDF regardless of aflibercept intervention. Thus, immunohistochemistry of retinas treated with aflibercept did not show major changes in regulation of the endoplasmin or PEDF in specific retinal layers. Scale bar = 100 µm. Reaction color: brown. Abbreviations: NFL: nerve fiber layer; GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer; PS: photoreceptor segments.