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Subcellular colocalization assays of CLN (5 μM) in the HeLa cells under hypoxic conditions. The green channel (Mito-Tracker Green) was excited at 488 nm, the blue channel (Hoechst 33342) was excited at 405 nm, and the red channel (CLN) was excited at 640 nm ((A) Scale bar = 10 μm; (B) Scale bar = 15 μm).
