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Cellular ROS levels of CLN (5 μM) in the 4T1 and HeLa cells employing a 5-μM 2′,7′-dichlorofluorescein diacetate (DCFH-DA) probe. The laser used a 660 nm (200 mW/cm2) laser light and 5 mM N-acetyl-L-cysteine (NAC) as reactive oxygen species scavenger (scale bar = 100 μm).
