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. 2022 May 31;11(11):1472. doi: 10.3390/plants11111472

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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RNA in situ hybridization of PnIAA30 transcripts in ovaries from sexual (Q4188) and apomictic (Q4117) P. notatum at anthesis. (a–f) Hybridization with the PnIAA30 antisense probe of sexual (a–c) and apomictic (d–f) genotypes. Notably, in the apomictic ovule, a proembryo showed almost no PnIAA30 hybridization signal ((e,f), adjacent sections of the same ovule). (g–k) Hybridization with PnIAA30 sense probe in sexual (g–i) and apomictic (j,k) ovaries. (l) DIC reference image for apomictic ovules at anthesis bearing multiple embryo sacs, parthenogenetic embryos and unfertilized polar nuclei. an: antipodal cells, ea: egg apparatus ec: egg cell, pn: polar nuclei, pe: pro-embryo, sy: synergid cells. Bars: 10 µm.