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. 2022 Jun 9;18(6):e1010576. doi: 10.1371/journal.ppat.1010576

Fig 2. ChIP-qPCR analyses of HBV cccDNA-associated active epigenetic markers in the presence and absence of HBx.

Fig 2

HBx-positive HBV cell line HepAD38 and HepBHAe82 and HBx-null HBV cell line HepBHAeΔx67 were induced in the absence of tet for 14 days. The association of (A) acetylated lysine 27 of histone H3 Lysine 27 (H3K27ac), (B) trimethylated lysine 4 of histone H3 (H3K4me3), (C) pan crotonylated lysine (Pan-Kcr), and (D) Phosphorylated C-terminal domain of RNA polymerase II (RNAPII pho-CTD) with cccDNA was analyzed by ChIP-qPCR and presented in percentage (%) of input (mean ± SEM, n = 3). *p<0.05, **p<0.01, ***p<0.001.