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. 2022 Jun 9;18(6):e1010576. doi: 10.1371/journal.ppat.1010576

Fig 3. ChIP-qPCR analyses of HBV cccDNA-associated repressive markers/factors in the presence and absence of HBx.

Fig 3

HBx-positive HBV cell line HepAD38 and HepBHAe82 and HBx-null HBV cell line HepBHAeΔx67 were induced in the absence of tet for 14 days. The association of cccDNA with (A) trimethylated lysine 27 of histone H3 (H3K27me3), a repressive histone PTM, (B) trimethylated lysine 9 of histone H3 (H3K9me3), a repressive histone PTM, and (C) Smc6, a reported host restriction factor of HBV cccDNA, was analyzed by ChIP-qPCR and presented in percentage (%) of input (mean ± SEM, n = 3). *p<0.05, **p<0.01.