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. 2022 Jun 9;18(6):e1010576. doi: 10.1371/journal.ppat.1010576

Fig 5. HMGB1 is highly enriched on cccDNA in the absence of HBx.

Fig 5

(A) HepAD38, HepBHAe82, and HepBHAeΔx67 cells were induced for HBV production for 14 days. The expression of endogenous HMGB1 was detected by Western blot, β-actin served as a loading control. The association of HMGB1 with cccDNA was analyzed by ChIP-qPCR and presented in percentage (%) of input (mean ± SEM, n = 3). (B) HepG2-NTCP cells were infected with wt HBV or HBVΔx for 10 days and subjected to the same analyses as aforementioned in (A). *p<0.05, **p<0.01.