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HepBHAeΔx67-shControl and HepBHAeΔx67-shHMGB1 cells were cultured in tet-free medium to induce HBV replication for 14 days, the association of (A) H3K27ac, (B) H3K27me3, and (C) RNAPII pho-CTD with cccDNA was analyzed by ChIP-qPCR and plotted in percentage (%) of input (mean ± SEM, n = 3). *p<0.05, ***p<0.001.
