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HepG2-NTCP-shControl and HepG2-NTCP-shHMGB1 cells were infected by HBVΔx at 500 vge/cell for 6 days. (A) Intracellular HBV pC mRNA was quantified by qPCR and normalized to GAPDH mRNA and HBV cccDNA (mean ± SEM, n = 3); (B) The levels of HBeAg in supernatant were detected by ELISA and expressed as fold changes (mean ± SEM, n = 3). **p<0.01, ***p<0.001.
