FIG. 4.

Cointegrate formation of IS91-based plasmids. Plasmid pSU2600 (host range X) with a complete IS91 can form a cointegrate plasmid with a new host range (X+Y) after recombination with IS91R of the suicide plasmid pJBS620 (host range Y). Oligonucleotides Cat3′, Cat5′, and ETB2 for PCR amplification and sequencing primer ETA2seq are shown as small arrows. The target sequences at the ends of IS91 and IS91R and relevant restriction sites are indicated.