TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or relevant characteristics | Toxin status | Source or reference |
|---|---|---|---|
| Human ETEC isolates | |||
| S1-008C | O6:H− | LT+ ST+ | P. Echeverria |
| S2-018B | O78:H− | LT+ ST+ | P. Echeverria |
| S6-044A | O25:H− | LT+ ST− | P. Echeverria |
| 102-34344F | NDa | LT+ ST+ | J. Hacker |
| K1 164/82 | O148:H28 | LT+ ST− | H. Karch |
| K2 297/87 | O25:H42 | LT+ ST− | H. Karch |
| K3 G1253 | O147:H19:K88 | LT+ ST− | H. Karch |
| K4 117/86 | O6:H− | LT+ ST+ | H. Karch |
| Porcine ETEC isolate K5 284/97 | ND | LT+ ST− | H. Karch |
| E. coli K-12 laboratory strains | |||
| MC4100 | F−araD139 Δ(argF-lac)U169 rpsL150 (Strr) relA1 flbB5301 deoC1 ptsF25 rbsR | 6 | |
| MC4100 recA | F−araD139 Δ(argF-lac)U169 rpsL150 (Strr) relA1 flbB5301 deoC1 ptsF25 rbsR recA::kan | J. J. Mekalanos | |
| MC4100 λpir | F−araD139 Δ(argF-lac)U169 rpsL150 (Strr) relA1 flbB5301 deoC1 ptsF25 rbsR λpir | 10 | |
| MC4100 λpir recA | MC4100 λpir recA::kan | This work | |
| XL1-Blue | F′::Tn10 proA+B+ lacIq Δ(lacZ)M15/recA1 endA1 gyrA96 (Nalr) thi hsdR17(rK− mK+) supE44 relA1 lac | New England Biolabs | |
| Plasmids | |||
| pACYC184 | Rep(p15A) Cmr Tetr | 7 | |
| pSU2600 | Rep(p15A) IS91 Cmr | 28 | |
| pCVD442 | Rep(R6K) mobRP4 sacB Apr | 9 | |
| pJBS620 | pCVD442 IS91R eltAB Apr | This work |
a
ND, not determined.