TABLE 2.
Oligonucleotides used in this study
| Name | Sequence (5′→3′) | Purpose |
|---|---|---|
| ETAseq | TCAGCACGGTATAATTTGTCGCCA | Sequencing of flanking regions of eltAB |
| ETA2seq | AAGCGATAAAGGAAGAGTGC | |
| ETBseq | GCCATTGAAAGGATGAAGGACACA | |
| IS91L | TGTCATTGTCTTTCAGGTAGTT | PCR amplification of the IS91 element |
| IS91R | TTTTTCTCACCGTCTCCGTTAT | |
| IS91R2 | TTGTCGACCATATGAGCGGAAA | |
| ETA1 | CCGGATTGTCTTCTTGTATGATA | PCR amplification of the enterotoxin genes eltAB |
| ETB2 | GGTCTCGGTCAGATATGTGATTC | |
| S6LSalI | TGAAAAACCAGTCGACACCATTGTCTATCG | PCR amplification of the IS91R eltAB fragment from isolate S6 |
| S6RSphI | TTCAAATGCAGCATGCGGCTCATTA | |
| Cat5′ | AACTGCAGTACGTAGCACCTCAAAAACACCATCATACAC | Detection of cointegrate formation together with ETB2 by PCR |
| Cat3′ | AATACGTACTGCAGCAGGCGTTTAAGGGCACCAATAACT | Detection of cointegrate formation together with ETB2 by PCR |