Table 5.
The compositions and detecting wavelengths of enzyme reactions.
| Enzyme | Substrate | Indicator | Detected wavelength |
|---|---|---|---|
| ≥10 unit/mg (type VII), porcine pancreatic α-amylase |
p-nitrophenyl-α-d-maltopentaoside | 405 nm | |
| ≥10 U/mg protein (type I), Saccharomyces cerevisiae α-glucosidase |
p-nitrophenyl-α-d-glucopyranoside | ||
| ≥700 unit/mg (type VII), Candida rugosa lipase |
DNPDB | DTNB | 412 nm |
| 1000 units/mg, Electrophorus electricus AChE |
acetylthiocholine | ||
| ≥10 units/mg, equine serum BChE |
butyrylthiocholine | ||
DNPDB: 5–5′-dithiobis (2-nitrobenzoic-N-phenacyl-4,5-dimethyyhiazolium bromide; DTNB: 5,5′-dithiobis (2-nitrobenzoic acid); AChE: acetylcholinesterase; BChE: butyrylcholinesterase.