Figure 5.

Immunoblot analysis of the proteins involved in photosynthesis in wheat treated with the plant-derived smoke solution under flooding stress. Proteins extracted from leaves of wheat seedlings were separated on SDS-polyacrylamide gel by electrophoresis and transferred onto membranes. The membranes were cross-reacted with anti-RuBisCO activase, the RuBisCO large subunit, and the RuBisCO small subunit antibodies. A staining pattern with Coomassie-brilliant blue was used as a loading control (Figure S1). The integrated densities of the bands were calculated using ImageJ software. The data are presented as mean ± SD from 3 independent biological replicates (Figure S2). Asterisks indicate significant changes in the relative intensity of signal band in the plant-derived smoke solution under flooding compared to only flooding according to the Student’s t-test (**, p < 0.01; *, p < 0.05).