Figure 7.

Immunoblot analysis of the proteins involved in glycolysis in wheat treated with the plant-derived smoke solution under flooding stress. Proteins extracted from the leaves and roots of wheat seedlings were separated on SDS-polyacrylamide gel by electrophoresis and transferred onto membranes. The membranes were cross-reacted with anti-FBPA, TPI, and GAPDH antibodies. A staining pattern with Coomassie-brilliant blue was used as a loading control (Figure S1). The integrated densities of bands were calculated using ImageJ software. The data are presented as mean ± SD from 3 independent biological replicates (Figures S3–S5). Asterisks indicate significant changes in the relative intensity of the signal band in the plant-derived smoke solution under flooding compared to only flooding according to the Student’s t-test (**: p < 0.01).