Figure 1.

Cancer-associated stimuli increase lipolysis in 3T3-L1 adipocytes, as measured by glycerol and free fatty acid (FFA) release. Mature 3T3-L1 adipocytes were treated with (A) TNF-α (16 h), (B) IL-6 (16 h), (C) NIH-3T3 fibroblast-conditioned media (FCM) or PANC-1 cancer conditioned media (CCM) (8 h), n = 3/group. All data presented were mean ± S.E.M. Treatments with different letters are statistically different (p < 0.05, Tukey’s post-hoc test following ANOVA). Where only two treatments were analyzed, p values were calculated using Student’s t-test. ** p < 0.01; *** p < 0.001.