Figure 3.

Alterations in expression of lipolytic proteins and genes by piceatannol and cancer-associated stimuli. Mature 3T3-L1 adipocytes were pre-treated with lipolytic stimuli for 16 h followed by 8 h stimuli-piceatannol (50 mM) co-treatment (A,B,D,E) or 16 h stimuli-piceatannol co-treatment only (C). These cells were subjected to total RNA isolation to determine the mRNA levels of genes involved in lipolysis (adipose triglyceride lipase (ATGL), comparative gene identification-58 (CGI-58), hormone-sensitive lipase (HSL), and Perilipin) (A,B). Signals were normalized to β-actin (n = 3). The expression levels of ATGL, CGI-58, pHSL^ser660, phosphorylated signal-transducer-and-activator-of-transcription 3 (pSTAT3), and STAT3 in these cells were determined by Western blot analysis using β-actin as a loading control (C,D,E). Values represent relative density of pSTAT3/total STAT3 from three blots. Treatments with different letters are statistically different, as determined by ANOVA followed by Tukey’s post-hoc analysis (p < 0.05). All data presented were mean ± S.E.M.