Table 3.
Number of milk samples collected at weaning (68.02 ± 5.8 d) and post-weaning (71.6 ± 5.8 d) within weaning treatment and estimated species frequency (95% confidence interval) within culture-positive samples identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)
Weaning | Post-weaning | |||||
---|---|---|---|---|---|---|
PENN1 | FAST2 | COMBO3 | PENN | FAST | COMBO | |
Item | ||||||
No. of samples | 14 | 9 | 11 | 15 | 13 | 13 |
No. of positive (%) | 5 (36%) | 2 (22%) | 7 (64%) | 3 (20%) | 3 (23%) | 3 (23%) |
Bacteria4 | ||||||
Bacillus spp.5 | 0.80 [0.28, 0.99] | 1.00 [0.16, 1.00] | 1.00 [0.59, 1.00] | – | – | 0.33 [0.01, 0.91] |
Mannheimia haemolytica | 0.20 [0.01, 0.72] | – | 0.43 [0.10, 0.82] | – | – | 0.33 [0.01, 0.91] |
Staphylococcus aureus | 0.20 [0.01, 0.72] | – | – | 0.33 [0.01, 0.91] | 0.33 [0.01, 0.91] | – |
CoNS6 | 0.20 [0.01, 0.72] | 0.50 [0.01, 0.99] | 0.29 [0.04, 0.71] | 0.67 [0.09, 0.99] | 0.67 [0.09, 0.99] | 0.67 [0.09, 0.99] |
S. capitis | – | – | 0.14 [0.00, 0.58] | – | – | – |
S. chromogenes | 0.20 [0.01, 0.72] | – | – | – | – | – |
S. epidermidis | – | – | – | – | – | 0.33 [0.01, 0.91] |
S. equorum | 0.20 [0.01, 0.72] | 0.5 [0.01, 0.99] | – | 0.67 [0.09, 0.99] | – | 0.33 [0.01, 0.91] |
S. vitulinus | – | – | – | – | 0.33 [0.01, 0.91] | – |
S. xylosus | – | – | 0.14 [0.00, 0.58] | – | 0.33 [0.01, 0.91] | – |
Streptococcusspp. | 0.20 [0.01, 0.72] | – | 0.14 [0.00, 0.58] | – | – | 0.33 [0.01, 0.91] |
Str. gallolyticus | 0.20 [0.01, 0.72] | – | 0.14 [0.00, 0.58] | – | – | 0.33 [0.01, 0.91] |
PENN, ewes received an intramuscular injection of penicillin at weaning.
FAST, ewes had restricted feed access 48 h prior to and 72 h post-weaning.
COMBO, ewes were administered penicillin at weaning and had restricted feed access 48 h prior to and 72 h post-weaning.
Only bacteria genera or species identified at a frequency > 0.05 within weaning treatment by day are presented.
Bacillus spp. identified include B. altitudinis, B. atrophaeus, B. cereus, B. circulans, B. clausii, B. licheniformis, B. megaterium, B. pumilus, B. safensis, B. subtilis, and B. thuringiensis.
CoNS, coagulase-negative staphylococci.