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. Author manuscript; available in PMC: 2022 Jun 9.
Published in final edited form as: Cell Rep. 2018 May 1;23(5):1387–1398. doi: 10.1016/j.celrep.2018.04.009

Figure 5. Delayed ER-to-Golgi Trafficking in Selenof KO MEFs.

Figure 5.

(A) Immunofluorescence micrographs of WT, heterozygous, and Selenof KO MEFs expressing adenoviral ts045-VSVG-EGFP and a Golgi marker. Representative images show the overlapping area where the VSVG-EGFP is retained in the ER-to- Golgi area, 10 min after the cells were transferred from the restrictive temperature (40°C) to the permissive temperature (32°C). Scale bars: 10 mm.

(B) Quantification of VSVG fluorescence retained in the ER-to-Golgi area. Three cell lines per each genotype and more than 10 cells per each field were evaluated. Immunofluorescence microscopy images were analyzed by ImageJ. *p < 0.05; **p < 0.01.