Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Cricetulus griseus) | CHO-K1 (with Human artificial chromosome) | Oshimura Lab, Yamaguchi et al., 2011 | ||
| Cell line (Homo sapiens) | K562 (lymphoblast, adult leukemia) | ATCC | CCL-243 | |
| Cell line (Homo sapiens) | HEK293T (epithelial, embryo kidney) | ATCC | CRL-3216 | |
| Recombinant DNA reagent | pSL007_PB_pCMV-H2B-mIFP-T2A-rTetR-ratKRAB-zeo (plasmid) |
This paper | RRID: Addgene_179438 | Piggybac rTetR-CR: rat KRAB |
| Recombinant DNA reagent | pLB62_PB_pGK-H2B-mIFP-T2A-rTetR-humanKRAB-zeo (plasmid) | This paper | RRID: Addgene_179439 | Piggybac rTetR-CR: human KRAB |
| Recombinant DNA reagent | pLB37_PB_pGK-H2B-mIFP-T2A-rTetR-HDAC4-zeo (plasmid) | This paper | RRID: Addgene_179440 | Piggybac rTetR-CR: human HDAC4 |
| Recombinant DNA reagent | pSL006_phiC31_neo-5xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry (plasmid) | This paper | RRID: Addgene_179425 | NS reporter phiC31 |
| Recombinant DNA reagent | pMH010_phiC31_neo-5xTetO-pEF-H2B-mCitrine-1.2kb,lambda-pRSV-H2B-mCherry (plasmid) | This paper | RRID: Addgene_179427 | 1.2 kb reporter phiC31 |
| Recombinant DNA reagent | pSL008_phiC31_neo-5xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry (plasmid) | This paper | RRID: Addgene_179426 | 5 kb reporter phiC31 |
| Recombinant DNA reagent | pSL011_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry (plasmid) | This paper | RRID: Addgene_179428 | NS reporter AAVS1 |
| Recombinant DNA reagent | pSL012_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry (plasmid) | This paper | RRID: Addgene_179429 | 5 kb reporter AAVS1 |
| Recombinant DNA reagent | pMH002_phiC31_neo-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry (plasmid) | This paper | RRID: Addgene_179430 | Single cHS4 (SH) reporter phiC31 |
| Recombinant DNA reagent | pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry (plasmid) |
This paper | RRID: Addgene_179431 | Single core cHS4 (SC) reporter AAVS1 phiC31 |
| Recombinant DNA reagent | pMH004_phiC31_neo-HS4-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry (plasmid) |
This paper | RRID: Addgene_179432 | Double cHS4 (DH) reporter phiC31 |
| Recombinant DNA reagent | pMH005_phiC31_neo-core-5xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry (plasmid) |
This paper | RRID: Addgene_179433 | Double core cHS4 (DC) reporter phiC31 |
| Recombinant DNA reagent | pMH011_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry (plasmid) | This paper | RRID: Addgene_179434 | Single cHS4 (SH) reporter AAVS1 |
| Recombinant DNA reagent | pMH012_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry (plasmid) | This paper | RRID: Addgene_179435 | Single core cHS4 (SC) reporter AAVS1 |
| Recombinant DNA reagent | pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry (plasmid) | This paper | RRID: Addgene_179436 | Double cHS4 (DH) reporter AAVS1 |
| Recombinant DNA reagent | pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry (plasmid) | This paper | RRID: Addgene_179437 | Double core cHS4 (DC) reporter AAVS1 |
| Antibody | Anti-rabbit IgG H&L chain (guinea pig, polyclonal) | Antibodies-Online | Cat #ABIN101961 | CUT&RUN (1:100) |
| Antibody | Anti-H3ac (rabbit, polyclonal) | Active Motif | Cat #39,139 | CUT&RUN (1:100) |
| Antibody | Anti-H3K4me3 (rabbit, polyclonal) | Active Motif | Cat #39,159 | CUT&RUN (1:100) |
| Antibody | Anti-H3K27me3 (rabbit, monoclonal) | Cell Signaling | Cat #9,733 S | CUT&RUN (1:50) |
| Peptide, recombinant protein | pA-MNase | Henikoff Lab | ||
| Commercial assay or kit | CUTANA ChIC/CUT&RUN Kit | EpiCypher | Cat #14 – 1048 | CUT&RUN |
| Chemical compound, drug | Doxycycline | Tocris Bioscience | 4,090 | |
| Chemical compound, drug | Tazemetostat-6438 | Selleck Chemicals | S7128 | EZH2 inhibitor |
| Software, algorithm | MACKtrack | Taylor, 2019 | https://github.com/brookstaylorjr/MACKtrack |