Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 31;61(1):87. doi: 10.3892/ijo.2022.5377

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Huang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Knockdown of CDCA5 induces cell cycle arrest in the G2/M phase. (A) Knockdown of CDCA5 increased the cell population in the G2 phase, but decreased the number of cells in the G1 phase, as shown by flow cytometric analysis in 786-O and SN12-PM6 cells. (B) Immunostaining analysis using the mitotic marker p-histone H3 (Serine 10; red) revealed a marked decrease in the number of positive cells in the shCDCA5 group. Nuclear DNA (blue) was stained with DAPI. (C) Western blot analysis of cyclin B1 in both 786-O and SN12-PM6 cell lines following the knockdown of CDCA5. Scale bars, 50 µm. ^*P<0.05, ^**P<0.01, ^***P<0.001 and ^****P<0.0001 vs. negative control. CDCA5, cell division cycle-associated 5; NC, negative control.