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. 2022 May 31;61(1):87. doi: 10.3892/ijo.2022.5377

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Copyright: © Huang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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CDCA5 knockdown induces the DNA damage response. (A) Immunofluorescence staining revealed increased γ-H2AX foci (red) formation in 786-O cells following CDCA4 knockdown and an increased percentage of γ-H2AX-positive cells. (B) γ-H2AX foci (red) formation increased in SN12-PM6 cells following CDCA4 knockdown and the percentage of γ-H2AX-positive cells also increased. (C) Western blot analysis of proteins related to the DNA damage response following CDCA4 knockdown in both 786-O and SN12-PM6 cell lines. Scale bars, 5 µm. Data are presented as the mean ± standard deviation. ^**P<0.01 and ^****P<0.0001 vs. negative control. CDCA5, cell division cycle-associated 5; NC, negative control; γ-H2AX, γ-H2A histone family member X; BRCA1, breast cancer type 1.