Table 13. Digestion of pCR-II-Blunt-TOPO-Donor plasmid.
| KRAS | NRAS | |
|---|---|---|
| pCR-II-Blunt-TOPO-Donor (1 μg/μL) | 20 μL | 20 μL |
| CutSmart buffer 10× | 5 μL | 5 μL |
| EcoRI-HF (10 U/μL) | 3 μL | 3 μL |
| Nuclease free water | 22 μL | 22 μL |
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