Table 20. Ligation set up for the generation of Grb2 donor DNA.

0.1 pmol of 5HA 50 ng	0.8 μL
0.1 pmol of mNG 50 ng	0.9 μL
0.1 pmol of 3HA 50ng	1.1 μL
0.1 pmol of digested vector 185 ng	2 μL
HIFi DNA assembly master mix	10 μL
Nuclease free water	5.2 μL