Table 2.
Oligonucleotides used for gene expression analysis, genotyping, and sex determination.
| Gene | NCBI number | Primer sequence (5′→3′) | Base pairs |
|---|---|---|---|
| Housekeeping genes | |||
| β-Actin | NM_007393.5 | For: CCTCTATGCCAACACAGTGC | 206 |
| Rev: CCTGCTTGCTGATCCACATC | |||
| Gapdh | XM_011241214.1 | For: ACAACTCACTCAAGATTGTCAGCA | 121 |
| Rev: ATGGCATGGACTGTGGTCAT | |||
|
| |||
| Angiogenesis | |||
| hsFLT1 | XM_017020485.1 | For: AATCATTCCGAAGCAAGGTG | 221 |
| Rev: TTTCTTCCCACAGTCCCAAC | |||
| (s)Flt-1 | NM_001363135.1 | For: TATAAGGCAGCGGATTGACC | 159 |
| Rev: TCATACACATGCACGGAGGT | |||
| Flk-1 | NM_001363216.1 | For: GGCGGTGGTGACAGTATCTT | 162 |
| Rev: GTCACTGACAGAGGCGATGA | |||
| Flt-4 | NM_008029.3 | For: GTGGCTGTGAAGATGCTGAA | 199 |
| Rev: TGACACGCAAGAAGTTGGAG | |||
| Plgf | XM_011244016.1 | For: CGTCCTGTGTCCTTCTGAGT | 200 |
| Rev: CCTCTTCCTCTTCCCCTTGG | |||
| Vegfa | NM_001025257.3 | For: CAGGCTGCTGTAACGATGAA | 140 |
| Rev: GCATTCACATCTGCTGTGCT | |||
| Vegfb | NM_011697.3 | For: AACACAGCCAATGTGAATGC | 157 |
| Rev: GGAGTGGGATGGATGATGTC | |||
| Vegfc | NM_009506.2 | For: CAAGGCTTTTGAAGGCAAAG | 159 |
| Rev: TCCCCTGTCCTGGTATTGAG | |||
| Vegfd | NM_001308489.1 | For: CAACAGATCCGAGCAGCTTC | 155 |
| Rev: AAAGTTGCCGCAAATCTGGT | |||
|
| |||
| Hypoxia marker | |||
| HO-1 | NM_010442.2 | For: CACGCATATACCCGCTACCT | 175 |
| Rev: CCAGAGTGTTCATTCGAGCA | |||
|
| |||
| Cell adhesion markers | |||
| Cd31 | NM_008816.3 | For: ATGACCCAGCAACATTCACA | 200 |
| Rev: CACAGAGCACCGAAGTACCA | |||
| Cdh5 | NM_009868.4 | For: TCACCATTGAGACAGACCCC | 233 |
| Rev: TGGCAGCTTGAAGTGGTAGA | |||
| Ocln | NM_001360538.1 | For: ACAGTCCAATGGCCTACTCC | 162 |
| Rev: TACCATTGCTGCTGTACCGA | |||
| Cldn5 | NM_013805.4 | For: CTTTGTTACCTTGACCGGCG | 198 |
| Rev: CCCAGCTCGTACTTCTGTGA | |||
| Itgb1 | NM_010578.2 | For: GAGACATGTCAGACCTGCCT | 194 |
| Rev: TCCTTCTCCTTGCAATGGGT | |||
| Gja1 (Cx43) | NM_010288.3 | For: GAGGGGGTGAAGGAGTTTTC | 233 |
| Rev: TGCAATGAAGCTGAACATGAC | |||
|
| |||
| Brain cell type markers | |||
| Map-2 | NM_001310634.1 | For: CGACACAGAACAGAGGGAGT | 191 |
| Rev: TCTCAAACGGCTGGTGGTAT | |||
| NeuN | NM_001039167.1 | For: ACCCAAGCCTCAGTTAGCAT | 187 |
| Rev: GTGGAGGAGATGGGGTTTGA | |||
| Ngf | NM_013609.3 | For: CAGTGTCAGTGTGTGGGTTG | 206 |
| Rev: TGTGAGTCGTGGTGCAGTAT | |||
| Mbp | NM_001025251.2 | For: TGTTTCCTCTCAGAGCCCAG | 246 |
| Rev: AGCGACTCGATTCAGTGACA | |||
| Olig2 | NM_016967.2 | For: CCCCAGAACCCGATGATCTT | 206 |
| Rev: GGTGCTGGAGGAAGATGACT | |||
| Gfap | NM_001131020.1 | For: AAGGTTGAATCGCTGGAGGA | 205 |
| Rev: ACCACTCCTCTGTCTCTTGC | |||
| Anpep | NM_008486.3 | For: CAGGGCCTGTACATCTTCCA | 161 |
| Rev: TCAATGTTAGGTGCCGGAGT | |||
| Iba-1 | NM_001361501.1 | For: ATGCTGGAGAAACTTGGGGT | 191 |
| Rev: CCAGTTGGCCTCTTGTGTTC | |||
|
| |||
| Genotyping | |||
| hsFLT1 | NM_001159920.2 | For: CAAGGACGTAACTGAAGAGG | 465 |
| Rev: TTTCTTCCCACAGTCCCAAC | |||
| Col1a1 | For: CCATCCCAACAATACATCACA | 200 | |
| Rev: TGGTTTCTTTGGGCTAGAGG | |||
| rtTA | For: AAAGTCGCTCTGAGTTGTTAT | ||
| Rev-wt: GGAGCGGGAGAAATGGATATG | 650 | ||
| Rev-mut: GCGAAGAGTTTGTCCTCAACC | 340 | ||
|
| |||
| Sex determination | |||
| IL-3 | NM_010556.4 | For: GGGACTCCAAGCTTCAATCA | 544 |
| Rev: TGGAGGAGGAAGAAAAGCAA | |||
| Sry | NM_011564.1 | For: TGGGACTGGTGACAATTGTC | 402 |
| Rev: GAGTACAGGTGTGCAGCTCT | |||