Figure 1.

Schematics of TetTCR-SeqHD workflow. (a) DNA barcode for pMHC tetramer was synthesized with a 3’ polyA tail. Fluorophore labeled streptavidin conjugated with an oligonucleotide sequence complementary to the 5’ end of tetramer DNA barcode was then used to anneal to each unique tetramer DNA barcode to generate barcoded streptavidin. Barcoded streptavidin was further used to form tetramers with peptide loaded MHCs. (b) Each T cell sample was stained with a unique DNA-barcoded anti-CD50 antibody as a SampleTag, a panel of DNA-barcoded pMHC tetramers, and a panel of 59 DNA-barcoded antibodies. Stained cells were sorted and then loaded on BD Rhapsody single sell analysis platform for high-throughput and high-dimensional molecular profiling, including cognate antigen specificity, TCR sequences, targeted gene expression, and surface protein level.