Fig. 3. IGF1R deficiency depletes peripheral monocytes, bone marrow neutrophils and leukocyte counts in BALF, and attenuates the increase of serum IL6 and TNFα levels after experimental pulmonary metastasis.

A UBC-CreERT2;Igf1r^fl/fl and Igf1r^fl/fl female mice were treated with tamoxifen (TMX) for five consecutive days at four weeks (W) of age to induce Igf1r gene deletion (CreERT2). Then, mice were injected through the lateral tail vein with 1 × 10⁶ Lewis Lung Carcinoma (LLC) cells in PBS or an equal volume of PBS. Collection of blood, bone marrow (BM), BALF and lungs were performed on day (D) 14. B Total serum IL6 and TNFα levels from PBS- or LLC-challenged CreERT2 vs. Igf1r^fl/f mice (n = 5–6 mice per group). C Representative images showing May-Grünwald Giemsa (MGG) stained peripheral blood, BM and cytospin preparations (red and green arrowheads indicate neutrophils and macrophages, respectively) (Scale bars: 20 µm). Differential cell counts for eosinophils, neutrophils, monocytes and lymphocytes in peripheral blood (D), total cells and neutrophils in BM (E), and total cells, neutrophils, macrophages and lymphocytes, as well as total protein content in BALF (F) from PBS- or LLC-challenged CreERT2 vs. Igf1r^fl/fl mice (n = 5–8 mice per group). Data are expressed as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001 (Dunn–Sidak test for multiple comparisons).