Table 1.
Artificial guts used to estimate digestibility of nutrients in poultry.
| Measurement | Simulated portion of the gut | Assay condition |
Simulated animal/species | Reference | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Proventriculus/Gizzard (gastric phase) |
Small intestine (SI phase) |
||||||||||
| Commercial enzyme/chemical | Incubation time, min | T, °C | pH | Commercial enzyme/chemical | Incubation time, min | T, °C | pH | ||||
| Crude protein and dry matter | Gastric and SI phases | 240 | 37 | NA | 240 | 37 | NA | White Leghorn hen | Sakamoto et al. (1980) | ||
| Crude protein and dry matter | Gastric and SI phases | 60, 120, 180, 240 | 37 | NA | 60 (after 240 min in gastric phase), 120, 180, 240 | 37 | 6.5, 6.6, 6.7, 6.8, 6.9 | Broiler chicken | Clunies and Leeson (1984) | ||
| Starch | Gastric and SI phases | Pepsin, HCl | 30 | 37 | NA | Pancreatin, amyloglucosidase, invertase | 15–360 | 37 | 5.2 | Weurding et al. (2001) | |
| Starch | Gastric and SI phases | Pepsin, HCl | 30 | 41 | 2.5 | Pancreatin, amyloglucosidase, invertase | 15–360 | 41 | 5.6 | Broiler chicken | Ebsim (2013) |
| Starch | Gastric and SI phases | Pepsin, HCl | 30 | 42 | 2.5 | 15–240 | 41 | 5.6 | Broiler chicken | Karunaratne et al. (2018a) | |
| Starch | Gastric and SI phases | Pepsin, HCl | 30 | 42 | 2.5 | 15–240 | 41 | 5.6 | Broiler chicken | Karunaratne et al. (2018b) | |
| Crude protein | Gastric and SI phases | Pepsin, HCl | 30 | 41 | NA | Pancreatin | 180 | 41 | 7.5 | Broiler chicken | Bryan et al. (2018) |
| Crude protein | Gastric and SI phases | Pepsin, HCl | 30 | 41 | NA | Pancreatin | 180 | 41 | 7.5 | Broiler chicken | Bryan et al. (2019) |
| Crude protein | Gastric and SI phases | Pepsin, HCl | 30 | 41 | NA | Pancreatin | 180 | 41 | 7.5 | Broiler chicken | Bryan and Classen (2020) |
SI = small intestine; T = temperature.