Table 3.
Artificial guts used to assess the efficacy of carbohydrases and protease for poultry.
| Simulated portion of the gut | Assay condition |
Simulated animal/species | Reference | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Proventriculus/Gizzard (gastric phase) |
Small intestine (SI phase) |
|||||||||
| Commercial enzyme/chemical | Incubation time, min | T, °C | pH | Commercial enzyme/chemical | Incubation time, min | T, °C | pH | |||
| Gastric and SI phases | Pepsin, HCl | 45 | 40 | 2.5 | Pancreatin, Na Bicarbonate | 120 | 40 | 6.3–6.7 | Broiler chicken | Bedford and Classen (1993) |
| Crop, gastric and SI phases | Pepsin, HCl | 30 (crop) + 45 (gizzard) | 40 | 3.0 | Pancreatin | 60 | 40 | 6.5 | Broiler chicken | Tervila-Wilo et al. (1996) |
| Crop, gastric and SI phases | – | – | – | 3.0 | – | – | – | 7.0, 7.5 | Broiler chicken | Ao et al. (2008) |
| Crop pH 6.5 | ||||||||||
| Crop, gastric and SI phases | Pepsin, HCl | 30 (crop, pH not adjusted) + 45 (gizzard) | 40 | 3.0 | Pancreatin | 60 | 40 | 6.5 | Broiler chicken | Ao et al. (2010) |
| Crop, gastric and SI phases | Pepsin, HCl | 30 (crop, pH 6.0) + 45 (proventriculus/gizzard) | 40 | 3.0 | Pancreatin, Na Bicarbonate | 60 | 40 | 6.5 | Broiler chicken | Fengying et al. (2011) |
| Crop, gastric and SI phases | Pepsin, HCl | 30 (crop, pH 5.2) + 45 (gizzard) | 42 ± 1 | 1.4–2.0 | Pancreatin | 120 | 42 ± 1 | 6.4–6.8 | Broiler chicken | Suresh et al. (2019) |
| Gastric and SI phases | Pepsin, HCl | 20 | 42 | 3.5 | Pancreatin, Sodium bicarbonate | 80 | 42 | 6.0 | Broiler chicken | Morgan et al. (2019) |
SI = small intestine; T = temperature.