Table 5.
Simulated portion of the gut | Assay condition |
Simulated animal/species | Reference | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Proventriculus/Gizzard (gastric phase) |
Small intestine (SI phase) |
|||||||||
Commercial enzyme/chemical | Incubation time, min | T, °C | pH | Commercial enzyme/chemical | Incubation time, min | T, °C | pH | |||
Crop, gastric and SI phases | Pepsin, HCl | 30 (crop, pH 5.2) + 45 (proventriculus) | 40 | 1.4–2.0 | Pancreatin | 120 | 40 | 6.4–6.8 | Broiler chicken | Solís-Cruz et al. (2017) |
Crop, gastric and SI phases | Pepsin, HCl | 30 (crop, pH 5.2) + 45 (proventriculus) | 40 | 1.4–2.0 | Pancreatin | 120 | 40 | 6.4–6.8 | Broiler chicken | Zavala-Franco et al. (2018) |
Crop, gastric and SI phases | Pepsin, HCl | 60, 120 (crop, pH 4.5) + 30 (proventriculus) | 40 | 2.5 | Pancreatin | 60, 120 | 40 | 6.5 | Broiler chicken | Tso et al. (2019) |
Crop, gastric and SI phases | Pepsin, HCl | 60 (crop, pH 4.5–5.3) + 90 (proventriculus) | 40 | 1.9–3.7 | Pancreatin, Sodium bicarbonate, bile salt | 120 | 40 | 5.3–7.5 | Broiler chicken | Kolawole et al. (2019) |
SI = small intestine; T = temperature.