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. 2022 Mar 26;10:156–166. doi: 10.1016/j.aninu.2021.12.010

Table 5.

Artificial guts used to evaluate the efficacy of adsorbents against mycotoxins.

Simulated portion of the gut Assay condition
Simulated animal/species Reference
Proventriculus/Gizzard (gastric phase)
Small intestine (SI phase)
Commercial enzyme/chemical Incubation time, min T, °C pH Commercial enzyme/chemical Incubation time, min T, °C pH
Crop, gastric and SI phases Pepsin, HCl 30 (crop, pH 5.2) + 45 (proventriculus) 40 1.4–2.0 Pancreatin 120 40 6.4–6.8 Broiler chicken Solís-Cruz et al. (2017)
Crop, gastric and SI phases Pepsin, HCl 30 (crop, pH 5.2) + 45 (proventriculus) 40 1.4–2.0 Pancreatin 120 40 6.4–6.8 Broiler chicken Zavala-Franco et al. (2018)
Crop, gastric and SI phases Pepsin, HCl 60, 120 (crop, pH 4.5) + 30 (proventriculus) 40 2.5 Pancreatin 60, 120 40 6.5 Broiler chicken Tso et al. (2019)
Crop, gastric and SI phases Pepsin, HCl 60 (crop, pH 4.5–5.3) + 90 (proventriculus) 40 1.9–3.7 Pancreatin, Sodium bicarbonate, bile salt 120 40 5.3–7.5 Broiler chicken Kolawole et al. (2019)

SI = small intestine; T = temperature.