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. 2021 Nov 25;54(7):1761–1768. doi: 10.1007/s11255-021-03014-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Allicin inhibits the apoptosis of renal tubular epithelial cells induced by H/R in NRK-52E cells. A Cell apoptosis of NRK-52E cells was examined by flow cytometry. B The protein expression of Bax, cleaved caspase-3 and Bcl-2 was detected by western blot. GAPDH was as a loading control. C The mRNA expression of Bax, caspase-3 and Bcl-2 was evaluated by qRT-PCR analysis. (A) control group, (B) H/R group, (C) H/R group + 1 μM allicin group, (D) H/R + 3 μM allicin group, (E) H/R + 5 μM allicin group. *p < 0.05 vs. group A; ^#p < 0.05 vs. group B