Table 1.
Cryo-EM data collection, refinement, and validation statistics.
| TWIK1 pH 7.4 EMDB 25168 PDB 7SK0 |
TWIK1 pH 5.5 EMDB 25169 PDB 7SK1 |
|
|---|---|---|
| Data collection and processing | ||
| Magnification | 36,000 x | 36,000 x |
| Voltage (kV) | 200 | 200 |
| Micrographs (no.) | 5014 | 3217 |
| Electron exposure (e–/Å2) | 49.715 | 50.3523 |
| Defocus range (μm) | −0.6 to −2.0 | −0.6 to −2.0 |
| Super-resolution pixel size (Å) | 0.5685 | 0.5575 |
| Map pixel size (Å) | 1.137 | 1.115 |
| Symmetry imposed | C2 | C2 |
| Topaz particle images (no.) | 2,872,868 | 8,990,305 |
| Final particle images (no.) | 92,438 | 38,126 |
| Map resolution (Å) | 3.4 | 3.4 |
| FSC threshold | 0.143 | 0.143 |
| Refinement | ||
| Model resolution (Å) | 3.4 | 3.4 |
| FSC threshold | 0.143 | 0.143 |
| Map sharpening B factor (Å2) | −112.7 | −111.91 |
| Model composition | ||
| Nonhydrogen atoms | 4,153 | 4,297 |
| Protein residues | 514 | 524 |
| Ligands | 7 | 7 |
| B factors (Å2) | ||
| Protein | 147.3 | 143.13 |
| Ligand | 78.45 | 108.96 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.007 | 0.008 |
| Bond angles (°) | 1.101 | 1.067 |
| Validation | ||
| MolProbity score | 1.14 | 0.99 |
| Clashscore | 1.2 | 0.7 |
| Poor rotamers (%) | 0 | 0.43 |
| Ramachandran plot | ||
| Favored (%) | 95.69 | 96.15 |
| Allowed (%) | 4.31 | 3.85 |
| Disallowed (%) | 0 | 0 |