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. 2022 Jun 9;13:3191. doi: 10.1038/s41467-022-30928-x

Fig. 8. Translational activities of transcripts encoding factors related to maternal mRNA clearance in oocytes of 4-week or 14-month-old WT mice.

Fig. 8

Fluorescence microscopy (a), quantification of the fluorescent signals (b), and western blot (c) results showing the translation activities of the Cnot6l 3′-UTR in GV-arrested or MII-arrested oocytes of 4-week or 14-month-old mice. The GFP signal indicated translational activation of the Btg4 3’-UTR. An in vitro transcribed and polyadenylated mCherry mRNA was co-injected as a positive control. Fluorescence microscopy (d), quantification of the fluorescent signals (e), and western blot (f) results showing the translation activities of the Pabpn1l 3′-UTR in GV or MII oocytes of 4-week or 14-month-old mice. Fluorescence microscopy (g), quantification of the fluorescent signals (h), and western blot (i) results showing the translation activities of the Cnot7 3′-UTR in GV or MII oocytes of 4-week or 14-month-old mice. Scale bar = 100 µm (a, d, g). In b, e and h, data are presented as mean values ± SEM. P by two-tailed Student’s t-test. n = 15 oocytes for each experimental group.