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. 2022 Jun 10;13(6):543. doi: 10.1038/s41419-022-04993-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A H460 cells were transfected with pDs-Red-Mito for 24 h, followed by infection with Ad-GFP-LC3 and Ad-LacZ or Ad-LETM1 for 24 h. For the positive control, cells were treated with 30 μM CCCP for 8 h. These confocal images are representative of three independent experiments. Scale bars represent 10 μm or 5 μm. B Merged GFP-LC3 and Red-Mito signals from puncta in H460 cells were counted in each condition (n = 40). The results are displayed as mean ± SD and are representative of three independent experiments. *p < 0.05; **p < 0.01, versus the control Ad-LacZ samples, #p < 0.05, control Ad-LETM1 versus CCCP Ad-LETM1 sample. C H460 cells were transfected with mt-keima for 24 h, followed by infection with Ad-LacZ or Ad-LETM1 for 24 h. Next, these cells are treated CCCP for 8 h. These FACS analysis are representative of three independent experiments. The results are displayed as mean ± SD and are representative of three independent experiments. *p < 0.05; **p < 0.01, versus the control Ad-LacZ samples, #p < 0.05, control Ad-LETM1 versus CCCP Ad-LETM1 sample. D H460 cells were transfected with mt-keima for 24 h, followed by infection with Ad-LacZ or Ad-LETM1 for 24 h. Next, these cells are treated CCCP for 8 h. These cells are analyzed by confocal microscope. Scale bars represent 10 μm or 3.5 μm. E Ad-LacZ or Ad-LETM1-infected H460 cells were analyzed by transmission electron microscopy. Scale bars represent 0.5 μm. Arrowheads: mitochondria; N: nucleus; black arrows: autophagosomes; white arrows: ER.