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. 2022 Jun 9;13:3230. doi: 10.1038/s41467-022-30959-4

Fig. 2. Identification of the ion channel TRPM7 as a regulator of phagosome acidification.

Fig. 2

a Schematic showing the siRNA knockdown of candidate calcium channels in BMDMs. b Scatter plot showing the relationship between macrophage gene expression and relative effect on phagosomal acidification, depicted as mean Z-scores (n = 3) from siRNA channel screen; target genes are depicted by single point; derivation of Z-scores is described in Methods. Gene expression value is average expression level across four macrophage subsets (ImmGen). Gray box denotes quartile for highest gene expression and the most negative effect on the z-score of acidification. c Left panel shows overlaid histograms of acidification (CypHer5E fluorescence) in the Trpm7 knockdown (siTrpm7, purple) and control (siScrambled, black) cells. Right panel shows Mean CypHer5E mean fluorescent intensity (MFI) of CypHer5E. The bar graphs depict mean values and error bars reflect SD (n = 3 biological replicates). Data representative of n = 3 independent experiments. See also Supplementary Figs. 1 and 2. Source data are provided as a Source Data file, and statistical testing is described in “Statistics and Reproducibility”.