Fig. 4. Degradation of apoptotic cell cargo by peritoneal macrophages requires TRPM7.

a The experimental design to measure apoptotic cell clearance in vivo is schematized. b Quantification of engulfing peritoneal macrophages (F4/80+) in Trpm7^fl/fl (n = 8) and Trpm7^fl/fl LysM Cre (n = 7) mice during in vivo clearance of apoptotic Jurkat-GFP cells. c MFI of CypHer5E, which measures cargo acidification (left) and GFP, which is stably expressed in Jurkat-GFP cells (right). See also Supplemental Fig. 3 for gating strategy. d Quantification of cargo degradation by WT and KO peritoneal macrophages in vivo as depicted by the ratio of CypHer5E MFI to GFP MFI in Cargo+ peritoneal macrophages. Dots represent independent samples from individual mice in n = 3 independent experiments. Box charts display sample distribution and are described in Methods. Source data are provided as a Source Data file, and statistical testing is described in “Statistics and Reproducibility”.