Fig. 3. LFA-1 engagement promotes TCR-induced degranulation only within the same interface.
a–c OT-1 CTLs were activated by equal mixtures of stimulatory beads bearing the indicated proteins. a A schematic of the experiment. Dummy denotes beads coated with nonstimulatory pMHC (H-2Db-KAVY) alone. Trans and cis co-presentation of H-2Kb-OVA and ICAM-1 are indicated. b, c Graphs showing CTL degranulation (b) and CD69 expression (c), measured 90 min after CTL stimulation (N = 2 replicate experiments). Data points represent technical triplicate measurements from an individual experiment. Source data are provided as a Source Data file. Replicate studies are shown in Supplementary Fig. 6.