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. 2022 Jun 9;13:3222. doi: 10.1038/s41467-022-30809-3

Fig. 9. Talin, but not LFA-1, is required for CTL-mediated killing of B16F10 cells.

Fig. 9

a Representative histograms showing ICAM-1 expression in RMA-s (left) and B16F10 (right) cells (N = 2 replicate experiments). b Model of CTL-mediated killing of B16F10 cells that requires both the TCR and an unidentified integrin X. c OVA-loaded B16F10 target cells were mixed with OT-1 CTLs in the presence of LFA-1-blocking antibody (αLFA-1) or isotype control. Left, specific lysis, measured after 4 h (N = 2 replicate experiments). Right, Lamp1 exposure, measured after 90 min (N = 2 replicate experiments). d OVA-loaded B16F10 target cells were mixed with OT-1 Cas9 CTLs transduced with talin gRNA (TlnCR) or control nontargeting gRNA (NTCR). Left, specific lysis, measured after 4 h (N = 2 replicate experiments). Right, Lamp1 exposure, measured after 90 min (N = 2 replicate experiments). Data points in degranulation graphs represent technical triplicate measurements from an individual experiment. Data points in killing assays represent mean values calculated from technical triplicates. Source data are provided as a Source Data file. Replicate studies for (c, d) are shown in Supplementary Fig. 11.