TABLE 1.
PCR and sequencing primers for 16S rDNA
| Primera | Sequence | Priming siteb | Purpose |
|---|---|---|---|
| C16SF | 5′ TATGGAGAGTTTGATCCTGGCTCAG 3′ | 268 to 292 | PCR |
| B37 | 5′ TACGGYTACCTTGTTACGA 3′ | 1749 to 1731 | PCR |
| PUC1 | 5′ ACGCCAGGGTTTTCCCAG 3′ | Vector | Seqc |
| B12 | 5′ TGGCGCACGGGTGAGTAA 3′ | 366 to 383 | Seq |
| C16SF2 | 5′ GAGGATGATCAGTCACACTGGAA 3′ | 562 to 584 | Seq |
| C16SF3 | 5′ CAGGATTAGATACCCTGGTAGTC 3′ | 1022 to 1044 | Seq |
| C16SF4 | 5′ TCAGCTCGTGTCGTGAGATGT 3′ | 1308 to 1328 | Seq |
| M13Rev | 5′ CAGGAAACAGCTATGAC 3′ | Vector | Seq |
| C16SR3 | 5′ CGCGTATCTTCGAATTAAACCA 3′ | 1212 to 1191 | Seq |
| C16SR4 | 5′ GGTGATATCTACGGAATTTA 3′ | 950 to 931 | Seq |
| C16SR5 | 5′ GCAATATTCCCTACTGCTGCCT 3′ | 633 to 612 | Seq |
a
For primers B37 and B12, see the study by Fox et al. (2). Primers PUC1 and M13Rev were from Amersham Pharmacia Biotech. The other primers were our own constructs.
b
The numbers indicate the relative position on the 16S rDNA sequence of C. jejuni ATCC 43431 (GenBank accession no. Z29326).
c
Seq, sequencing.