TABLE 1.
Primera | Sequence | Priming siteb | Purpose |
---|---|---|---|
C16SF | 5′ TATGGAGAGTTTGATCCTGGCTCAG 3′ | 268 to 292 | PCR |
B37 | 5′ TACGGYTACCTTGTTACGA 3′ | 1749 to 1731 | PCR |
PUC1 | 5′ ACGCCAGGGTTTTCCCAG 3′ | Vector | Seqc |
B12 | 5′ TGGCGCACGGGTGAGTAA 3′ | 366 to 383 | Seq |
C16SF2 | 5′ GAGGATGATCAGTCACACTGGAA 3′ | 562 to 584 | Seq |
C16SF3 | 5′ CAGGATTAGATACCCTGGTAGTC 3′ | 1022 to 1044 | Seq |
C16SF4 | 5′ TCAGCTCGTGTCGTGAGATGT 3′ | 1308 to 1328 | Seq |
M13Rev | 5′ CAGGAAACAGCTATGAC 3′ | Vector | Seq |
C16SR3 | 5′ CGCGTATCTTCGAATTAAACCA 3′ | 1212 to 1191 | Seq |
C16SR4 | 5′ GGTGATATCTACGGAATTTA 3′ | 950 to 931 | Seq |
C16SR5 | 5′ GCAATATTCCCTACTGCTGCCT 3′ | 633 to 612 | Seq |
For primers B37 and B12, see the study by Fox et al. (2). Primers PUC1 and M13Rev were from Amersham Pharmacia Biotech. The other primers were our own constructs.
The numbers indicate the relative position on the 16S rDNA sequence of C. jejuni ATCC 43431 (GenBank accession no. Z29326).
Seq, sequencing.