Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 27;16:881480. doi: 10.3389/fnins.2022.881480

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Thrush, Vadukul and Aprile.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

(A) SDS-PAGE of samples taken throughout the purification process of 7–140 α-syn; crude extract (lane 1), flow through after passing the crude extract through the column five times (lane 2), flow through after washing the column with column buffer (lane 3), flow through after equilibrating the column with cleavage buffer (lane 4), compiled fractions containing the target protein after dialysis (lane 5), flow through after stripping the column with NaOH (lane 6), and the target protein purified by SEC (lane 7). The yellow arrows indicate the molecular weight (MW) of the fusion protein, while the purple arrow the one of 7–140 α-syn. (B) The deconvoluted ESI-MS spectrum of 7–140 α-syn. Theoretical mass 13,699.9 Da, experimental mass 13,698.0 Da. (C) The CD spectra of monomeric WT α-syn (blue) and 7–140 α-syn (purple). n = 3, error bars representing the standard deviation are shown as transparent bars.