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Changes of important genes in amino acid metabolism and target of tadalafil. (A) HCT116 cell viability after 10 μM tadalafil treatment for 24 h. (B) HT29 cell viability after 10 μM tadalafil treatment for 24 h. (C) SW620 cell viability after 10 μM tadalafil treatment for 24 h. (D) Cell viability analysis when pde5 was knocked down by siRNA. (E) Expression of GPT, GGT5 and TAT after tadalafil treatment with or without pde5 knockdown.
