Fig. 7.

SAM are potential protective microglia associated with ischemic stroke. (A) Violin plots showing the expression of Spp1 in homeostatic clusters 1 and 2 and SAM. (B) FACS analysis of Spp1 positive microglia population in the IL hemisphere of Prdx1+/+ (n = 5) and Prdx1−/− (n = 5) mice after tMCAO. (C) Representative IHC images for Spp1 positive SAM population in the ipsilateral hemisphere of Prdx1+/+ and Prdx1−/− mice. (D,F) Violin plots showing gene expression levels of the indicated genes in homeostatic clusters 1 and 2 and SAM. (E) Representative IHC images for Cd63 positive SAM population in the ipsilateral hemisphere of Prdx1+/+ mice. (G) Immunoblot analysis of Spp1 and Fth1 in CL and IL hemispheres of Prdx1+/+ and Prdx1−/− mice at 24 h after tMCAO (n = 4). Bar graph showing quantification of blotting images. (H) UMAP plots showing co-expression of Fth1 (red) and Cd63 (green). (I) FACS analysis showing Cd63 and Fth1 expression of Prdx1+/+ IL microglia and Prdx1−/− IL microglia the indicated conditions. Data are presented as Mean Fluorescence Intensity (MFI). (J) FACS analysis of Fth1, Cd63 double positive SAM population proportion and numbers in the IL hemisphere of Prdx1+/+ (n = 5) and Prdx1−/− (n = 5) mice. (K) Representative IHC images for Cd63, Fth1 double positive SAM population in the IL hemisphere of Prdx1+/+ and Prdx1−/− mice. (L) Representative IHC images for Fth1 and c-Caspase3 in infarct regions of Prdx1+/+ and Prdx1−/− microglia (IBA-1). All of mice were used at 24 h after tMCAO. Data were presented mean SEM and analyzed by 2-way ANOVA test or the unpaired two-tailed Student’s t-test, *P<0.05, **P<0.01, ***P<0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)